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The Iron and Heme Core provides analysis of metals, precursor porphyrins and heme. The core will measure activity of the enzymes responsible for heme biosynthesis. Analysis and quantification of heme and its precursors can be obtained for cell pellets, tissue, whole blood, urine, feces and other complex biological materials. Analysis of enzyme activity can be provided for cell pellets, tissue and blood.
The Iron and Heme Core is also prepared to assay for Total Iron Binding Capacity (TIBC) in serum, and to assay for human serum ferritin and mouse serum ferritin. Hepcidin will be assayed in the Metabolomics core (our sister CIHD core) by mass spectrometry.
The Iron and Heme Core will collect and distribute reagents from the iron and heme research community, including plasmids for the expression of proteins involved in heme biosynthesis. We expect that these reagents will begin to be available in August. Please watch this website for updates on availability.
Protocols for many of our assays are downloadable from this website in PDF form. Core staff will provide additional assay protocols and consultation upon request.