Postdoc opportunity

    Available postdoctoral positions in stem cells and immunology 9/30/21

    Up to two full-time postdoctoral fellowship positions are available (open until filled) at the University of Utah School of Medicine and Huntsman Cancer Institute in Salt Lake City, UT, to investigate gene regulatory circuitries underlying adaptive immunity. Talented and motivated candidates with a track record of success in gene regulation and immunology will be considered. Experience with basic molecular biological methods and cell culture is required. Experience with mouse models and bioinformatic analysis is ideal. Candidates must have PhD or equivalent training. The successful candidate will be able to work independently as well as in a collaborative setting. Starting salaries will be based on the NIH postdoc scale. Send cover letter, current CV and contact information for three potential references to Dr. Dean Tantin, dean.tantin@path.utah.edu. Be sure to include “PD position 1” in the subject line.

    The Tantin laboratory is committed to creating an inclusive work environment. All qualified individuals, including minorities, women, individuals with disabilities, and veterans are encouraged to apply.

    Dean Tantin, PhD
    Professor of Pathology
    Division of Microbiology&Immunology
    University of Utah School of Medicine
    Huntsman Cancer Institute
    2000 Circle of Hope, Research South 3707
    Salt Lake City, UT 84112-5550
    Email dean.tantin@path.utah.edu
    medicine.utah.edu/pathology/research/labs/dean-tantin/

    1 Postdoctoral Training Position Available  9/16/21

    Postdoctoral Position Available at the National Institutes of Health

     Iron Chaperones and the Distribution of Iron Cofactors within Cells

     

    Caroline C. Philpott, M. D.
    Genetics and Metabolism Section, LDB, NIDDK, NIH

    Postdoctoral position available to study the distribution and utilization of iron cofactors within mammalian cells and tissues. Iron is an essential nutrient for every cell in the human body, yet it can also be a potent cellular toxin. Iron is essential because enzymes that require iron co-factors (namely, heme, iron-sulfur clusters, mononuclear and diiron centers) are involved in virtually every major metabolic process in the cell. Iron deficiency continues to be the most common nutritional deficiency in the world, especially among children and women of childbearing age, where it causes anemia and impairs neurological development and function. Although the pathogenesis of anemia in iron deficiency is well understood, other manifestations of iron deficiency are not understood at the cellular or metabolic level. Iron overload is a feature of an increasing number of human diseases, including genetic disorders such as hereditary hemochromatosis, thalassemias, and Friedreich ataxia, as well as chronic inflammatory diseases of the liver, such as hepatitis C. Hundreds of iron, zinc, copper, and manganese proteins are expressed in human cells, yet little is known about the mechanisms by which these metalloproteins acquire their native metal ligands and avoid mis-metallation. We have made significant advances in understanding the delivery of iron to iron-dependent enzymes in the cytosol.

    We identified Poly rC-Binding Protein 1 (PCBP1) as an iron-binding protein that delivers iron to ferritin in human cells (Shi, et al. 2008, Science 320, 1207-10). This was the first description of a cytosolic iron chaperone – a protein that specifically binds iron ions and delivers them to target proteins through direct protein-protein interactions.  PCBP1 and its human paralogs are multifunctional adaptor proteins that also bind single-stranded DNA and RNA in a sequence-specific manner to regulate the fate of the nucleic acid. PCBP2, a human paralog of PCBP1, is independently required for the delivery of iron to ferritin. PCBP1 and PCBP2 deliver iron to additional families of target enzymes: the prolyl hydroxylases (PHDs) that regulate the degradation of hypoxia inducible factor 1 (HIF1) and deoxyhypusine hydroxylase (DOHH). Projects currently underway explore the roles of PCBPs in erythroid cell development, duodenal iron absorption, macrophage iron recycling, and the intersection of iron ion chaperones with the Fe-S cluster machinery. Mouse models of PCBP1 and PCBP2 deficiency have been developed and are revealing new functions of these proteins in maintaining iron homeostasis in mammals.

    We use the tools of cell biology, genetics and biochemistry to address questions about how cells and animals use iron. The postdoctoral position is fully funded, available immediately, and open to any motivated Ph.D./M.D. with less than five years of postdoctoral experience. NIH is an equal opportunity employer.

    Contact:

    Caroline C. Philpott, M. D.Chief, Genetics and Metabolism Section
    Liver Diseases Branch, NIDDK, NIH
    Bldg 10, Rm 9B-16
    10 Center Drive
    Bethesda, MD 20892-1800

    Web: http://irp.nih.gov/pi/caroline-philpott
    Phone: 301-435-4018
    Fax: 301-402-0491
    Email: carolinep@mail.nih.gov

    2 Postdoctoral Training Positions Available  5/14/21

    Postdoctoral positions in the

      Enns/Zhang Labs in the Department
    of Cell, Developmental, and Cancer Biology at Oregon Health &
    Science University, Portland, OR USA. Detail link

     

    Postdoctoral Training Position Available Fellowship   5/7/21 

    Bone Marrow Spatial Transcriptomics to Enhance In Vitro Platelet Production:
    The Moffitt and Cantor Laboratories at Boston Children’s Hospital/Harvard Medical School (HMS) are currently
    accepting applications for an entry-level joint post-doctoral training position. The Moffitt lab focuses on
    development and utilization of in situ single-molecule imaging methods to further understand tissue
    architecture, developmental signaling, and novel cell type identification. The Cantor lab specializes in
    hematopoiesis and platelet production. This joint project will develop and apply Multiplexed Error-robust
    Fluorescence In Situ Hybridization (MERFISH) to the mouse and human bone marrow to further understand
    how megakaryocytes generate platelets in the context of their native microenvironment. This knowledge will
    then be applied to enhance in vitro platelet production from induced pluripotent stem cells (hIPSCs) for
    transfusion purposes. The candidate will also interact with the Allon Klein’s laboratory at HMS to develop and
    correlate scRNA-seq and in situ transcriptomic datasets. The candidate will be jointly advised by Dr. Moffitt and
    Dr. Cantor and will have the opportunity to learn and develop a cutting-edge spatial transcriptomics tool while
    substantially advancing our understanding of bone micro-environment and its critical role in the important
    process of platelet production and stem cell biology.
    Candidates should have recently (within ~1 year) obtained a PhD or an MD/PhD degree in the fields of
    Computational Biology, Genomics, Molecular Biology, Cellular Biology, Biochemistry, Development, or
    Genetics. The candidate should have evidence of prior productive scientific work in the form of publications in
    peer-reviewed journals. Prior experience in computational biology, scRNAseq, and/or other “-omics” type
    approaches is strongly preferred. U.S. citizenship or Permanent Resident status (i.e., “Green Card” holders) is
    not necessary, but is favored.
    Interested candidates should send their Curriculum Vitae and a cover letter describing their background and
    research interests to jeffrey.moffitt@childrens.harvard.edu and alan.cantor@childrens.harvard.edu.
    Candidates should also arrange for two letters of recommendation, which may be requested. Jeffrey Moffitt, PhD

    Alan Cantor, MD, PhD
    Assistant Professor of Pediatrics Associate Professor of Pediatrics
    Program in Cellular and Molecular Medicine Division of Pediatric Hematology/Oncology
    Office: 617-713-8902 Office:617-919-2026
    Boston Children’s Hospital is an equal opportunity employer. Women and members of under-represented
    minorities are encouraged to apply

     

    Postdoc Fellow/Staff Scientist 5/6/21

    Location: Boston Children’s Hospital, Harvard Medical School

    Job Description:
    The Bauer laboratory seeks highly motivated post-doctoral research fellows and staff scientists to investigate
    therapeutic gene editing to address human diseases with unmet clinical needs and to explore the functional
    genomics of human hematopoiesis. We conduct studies ranging from technology development, target discovery,
    preclinical validation and first-in-human clinical application.
    We have identified regulatory elements that are subject to naturally occurring disease associated genetic variation
    and are critical determinants of fetal hemoglobin level and hemoglobin disorder clinical severity. We have
    developed methodologies for high-throughput and high-resolution functional evaluation of coding and noncoding
    genetic elements. We have demonstrated highly efficient and specific methods for nuclease and base editing of
    human hematopoietic stem and progenitor cells. We use molecular genetic, biochemical, and genome editing
    methodologies to perturb and observe blood cells. Our studies are meant to elucidate fundamental mechanisms
    of gene regulatory elements in their endogenous chromosomal environment, to explore determinants of blood cell
    development, homeostasis, and disease, and to define structure-function relationships of disease relevant protein
    complexes. A major motivation of our work is to translate findings into novel therapies for patients with blood
    disorders. We are advancing therapeutic gene editing from preclinical studies to first-in-human trials.
    Prior experience in gene editing/gene therapy, protein engineering, hematopoiesis, molecular and cell biology,
    genetics, biochemistry, systems biology, structural/chemical biology, and/or bioinformatics/computational biology
    is highly desirable. The candidate must be independent in scientific research and writing, self-motivated, ethical,
    team spirited, and must have exceptional laboratory, communication, organizational, and writing skills.
    Interested applicants should send a CV, cover letter, statement of research interest and contact information for
    three references via email to

    Daniel Bauer, MD PhD (daniel.bauer@childrens.harvard.edu), Division of
    Hematology/Oncology, Boston Children’s Hospital, Harvard Medical School, Boston, MA. Boston Children’s
    Hospital is one of the top pediatric research centers in the world, and a major research and teaching affiliate of
    Harvard Medical School. The Bauer Laboratory is also affiliated with the Dana-Farber Cancer Institute, Harvard
    Stem Cell Institute, and Broad Institute of Harvard and MIT.

     

     

     

     

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